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This research provides direct experimental evidence confirming that methotrexate functions as a competitive inhibitor of dihydrofolate reductase (DHFR). Understanding this specific mechanism is crucial for comprehending methotrexate's pharmacological actions and its implications in therapeutic applications, particularly in cancer treatment and autoimmune diseases.

Discover a direct and highly precise method for analyzing Methotrexate, Dichloromethotrexate, and their crucial 7-hydroxy metabolites directly within plasma samples. This advanced technique utilizes High Pressure Liquid Chromatography (HPLC) to ensure accurate quantification, providing essential data for pharmacokinetic studies and therapeutic drug monitoring.